abstract：:Phosphatidylinositol (PtdIns) and phosphatidylinositol 4-phosphate (PtdIns4P) kinase activities in plasma membranes isolated from canine left ventricle were partially characterized, and their sensitivity to a number of intracellular variables was established. PtdIns and PtdIns4P kinase activities were estimated by the...

journal_title：Lipids

authors： Kasinathan C,Xu ZC,Kirchberger MA

更新日期：1989-09-01 00:00:00

abstract：:The calcium ionophore A23187 stimulates release of free [3H]arachidonic acids from radiolabeled cultures of MEPM cells which are growing, but not from those which are confluent. However, when confluent MEPM cells are pretreated with EGF or PMA, release of [3H]arachidonic acids does occur in response to A23187. Since n...

journal_title：Lipids

authors： Chepenik KP

更新日期：1989-09-01 00:00:00

abstract：:A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted...

journal_title：Lipids

authors： Collins SE,Jackson MB,Lammi-Keefe CJ,Jensen RG

更新日期：1989-08-01 00:00:00

abstract：:A simple and sensitive method to analyze mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described. The method involves the oxidative conversion of the sterols with cholesterol oxidase, followed by high performance liquid chromatographic (HPLC) analysis. A C18 reversed phase column (3 microns, 75 X 4....

journal_title：Lipids

authors： Goh EH,Colles SM,Otte KD

更新日期：1989-07-01 00:00:00

abstract：:A new spectrophotometric assay for determining the activity of acylglycerol hydrolases (lipases, E.C. 3.1.1.3) was developed and optimized for yeast lipase (Candida cylindracea). Studies with porcine pancreatic lipase were also conducted and the influence of various detergents and divalent cations on the assay was eva...

journal_title：Lipids

authors： Rogel AM,Stone WL,Adebonojo FO

更新日期：1989-06-01 00:00:00

abstract：:The minimum requirement of linoleic acid to prevent effects of dietary C18 trans fatty acids on eicosanoid biosynthesis in rats was assessed. In a first experiment, six groups of animals were fed diets with a high content of trans fatty acids [20% of energy (en%)], and increasing amounts of linoleic acid (0.4 to 7.1 e...

journal_title：Lipids

authors： Zevenbergen JL,Haddeman E

更新日期：1989-06-01 00:00:00

abstract：:The hypolipidemic drug clofibrate is known to affect the hepatic transport of various organic anions including bilirubin, fatty acids and sulfobromophthalein. Changes in the rate of metabolism and/or intracellular transport have been claimed responsible for the effect. To evaluate these possibilities, the transport of...

journal_title：Lipids

authors： Sorrentino D,Weisiger RA,Bass NM,Licko V

更新日期：1989-05-01 00:00:00

abstract：:Male weanling rats were fed semi-synthetic diets high in saturated fat (beef tallow) vs high in linoleic acid (safflower oil) with or without high levels of alpha-linolenic acid (linseed oil) for a period of 28 days. The effect of feeding these diets on cholesterol content and fatty acid composition of serum and liver...

journal_title：Lipids

authors： Garg ML,Wierzbicki AA,Thomson AB,Clandinin MT

更新日期：1989-04-01 00:00:00

abstract：:The amount and composition of lysophosphatidate present in different rat tissues have been estimated by an internal standard method in which a synthetic unnatural isomer (1-heptadecanoyl-rac-glycerol-3-phosphate) was added to the total lipid extracts, and the fatty acid composition of purified lysophosphatidate was de...

journal_title：Lipids

authors： Das AK,Hajra AK

更新日期：1989-04-01 00:00:00

abstract：:In this study, we investigated the effect of various types of fats on heart lipid peroxidation status and on blood lipid parameters. Rats were fed either a low-fat diet (2.2% lard plus 2.2% corn oil), a corn oil diet (17%), a salmon oil diet (12.5%) supplemented with 4.5% corn oil, or a lard diet (15%) supplemented wi...

journal_title：Lipids

authors： Nalbone G,Leonardi J,Termine E,Portugal H,Lechene P,Pauli AM,Lafont H

更新日期：1989-03-01 00:00:00

abstract：:Fast atom bombardment-tandem mass spectrometry was used to identify molecular species of paf-acether (paf) produced by human polymorphonuclear neutrophils. Using this biological material, normal phase high performance liquid chromatography was necessary prior to the fast atom bombardment-tandem mass spectrometry step....

journal_title：Lipids

authors： Bossant MJ,Farinotti R,De Maack F,Mahuzier G,Benveniste J,Ninio E

更新日期：1989-02-01 00:00:00

abstract：:A systematic study of the influence of biological lipid peroxidation conditions on lipid hydroperoxide decomposition to thiobarbituric acid-reactive malondialdehyde is presented. A superoxide-dependent, iron-catalyzed peroxidation system was employed with xanthine oxidase plus hypoxanthine plus ferric iron-adenosine d...

journal_title：Lipids

authors： Janero DR,Burghardt B

更新日期：1989-02-01 00:00:00

abstract：:We examined the initial transport of a long-chain unsaturated fatty acid, linoleic acid, by brush border membrane vesicles isolated from rabbit small intestine. This preparation allowed us to examine the transport of linoleic acid across the brush border membrane without the effect of the unstirred water layer or cyto...

journal_title：Lipids

authors： Ling KY,Lee HY,Hollander D

更新日期：1989-01-01 00:00:00

abstract：:Normal phase high performance liquid chromatography methods are described for the separation of neutral lipid, fatty acid and five phospholipid classes using spectrophotometric detection at 206 nm. Separations were accomplished in less than 10 min for each lipid class. A mobile phase consisting of hexane/methyltertiar...

journal_title：Lipids

authors： Hamilton JG,Comai K

更新日期：1988-12-01 00:00:00

abstract：:Recently, lipases have been purified from mammalian, bacterial, fungal and plant sources by different methodologies. Purified lipases subsequently have been characterized for molecular size, metal binding capabilities, glycoside and phosphorus contents, and substrate specificities. Primary structures of several lipase...

journal_title：Lipids

authors： Antonian E

更新日期：1988-12-01 00:00:00

abstract：:The current state of knowledge concerning the absorption and transport of dietary fat with emphasis on long-chain polyunsaturated n-3 fatty acids in mammals is reviewed. It is apparent that long-chain polyunsaturated n-3 fatty acids, either as free acids or as part of triglycerides, are readily absorbed in the gut and...

journal_title：Lipids

authors： Nelson GJ,Ackman RG

更新日期：1988-11-01 00:00:00

abstract：:Racemic cis-9,10-epoxystearic acid was isolated from total lipids of human leukocytes. Identification of the epoxy acid was based mainly on gas liquid chromatographic-mass spectrometric analysis and on its chemical conversion into threo-9,10-dihydroxystearic acid. A mass spectrometric method for quantitative determina...

journal_title：Lipids

authors： Fahlstadius P

更新日期：1988-11-01 00:00:00

abstract：:After intravenous injection of liposomes prepared from egg yolk phospholipids into rabbits, the phospholipids were readily assimilated by the lipoproteins, and there were increases in the circulating levels of cholesterol and phospholipids. The increases in cholesterol level were mainly due to increases of free choles...

journal_title：Lipids

authors： Mendez AJ,He JL,Huang HS,Wen SR,Hsia SL

更新日期：1988-10-01 00:00:00

abstract：:Biosynthesis of peripheral nerve cholesterol was investigated by the in vivo and in vitro incorporation of [1-14C]-acetate into sciatic endoneurium of normal rats during development, degeneration and regeneration. Labeled sterols were rapidly formed (less than 10 min) within the endoneurial portion of sciatic nerve af...

journal_title：Lipids

authors： Yao JK

更新日期：1988-09-01 00:00:00

abstract：:The reaction products of alpha-tocopherol and its model compound, 2,2,5,7,8-pentamethylchroman-6-ol, during the autoxidation of methyl linoleate at 37 C were investigated. Two isomeric trimers were obtained as the major reaction products of alpha-tocopherol, and a trimer was obtained as that of its model chroman. The ...

journal_title：Lipids

authors： Yamauchi R,Kato K,Ueno Y

更新日期：1988-08-01 00:00:00

abstract：:Three groups of male rats were fed either a corn oil-enriched diet (17%, w/w), a salmon oil-enriched diet (12.5%) supplemented with corn oil (4.5%) or a low-fat diet (4.4%) for eight wk to investigate the possible relationships between dietary fatty acids and lipid composition, and prostaglandin E2 level and phospholi...

journal_title：Lipids

authors： Grataroli R,Léonardi J,Charbonnier M,Lafont R,Lafont H,Nalbone G

更新日期：1988-07-01 00:00:00

abstract：:The rare phospholipid fatty acids 3,7,11-trimethyldodecanoic (1), 5,9-hexadecadienoic (2) and 12-methyl-hexadecanoic (3) were identified in the marine spongeXestospongia muta. Branched fatty acids inX. muta accounted for 35% of the total fatty acid mixture. It was observed that the occurrence of the 5,9-hexadecadienoi...

journal_title：Lipids

authors： Carballeira NM,Maldonado L

更新日期：1988-07-01 00:00:00

abstract：:Two groups of volunteers had blood drawn for serum analysis of fatty acids. The first group was comprised of patients admitted to the hospital with possible myocardial infarction (MI). Blood was drawn at admission and at 12, 24 and 48 hr. These patients were subsequently divided into three groups, those with MI, those...

journal_title：Lipids

authors： Crofts JW,Ogburn PL Jr,Johnson SB,Holman RT

更新日期：1988-06-01 00:00:00

abstract：:A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic t...

journal_title：Lipids

authors： Dousset N,Negre A,Salvayre R,Rogalle P,Dang QQ,Douste-Blazy L

更新日期：1988-06-01 00:00:00

abstract：:A method has been developed for determination of individual long-chain fatty acyl-CoA esters from heart and skeletal muscle using high performance liquid chromatography (HPLC). The esters were extracted from freeze-clamped tissue of pig and rat hearts and rat skeletal muscle for analysis on a radially compressed C18 5...

journal_title：Lipids

authors： Molaparast-Saless F,Shrago E,Spennetta TL,Donatello S,Kneeland LM,Nellis SH,Liedtke AJ

更新日期：1988-05-01 00:00:00

abstract：:A 24-hr pretreatment of cultured human fibroblasts with trifluoperazine induced a marked increase in incorporation of saturated (stearic, palmitic) and unsaturated (oleic, arachidonic) fatty acids into phospholipids (1.5- to 2-fold for 5.10(-5) M trifluoperazine). Concomitantly, incorporation into cholesteryl esters w...

journal_title：Lipids

authors： Mazière C,Mazière JC,Mora L,Auclair M,Polonovski J

更新日期：1988-05-01 00:00:00

abstract：:Dietary fat and abnormal production of various prostanoids have been linked to various disease states of the large bowel, including cancer of the colon. Studies were conducted to determine the effect of dietary fat (beef tallow or corn oil) on the lipid composition and prostanoid production of the murine colon. Female...

journal_title：Lipids

authors： Robblee NM,Farnworth ER,Bird RP

更新日期：1988-04-01 00:00:00

abstract：:Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2-acyl-sn-glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene deriva...

journal_title：Lipids

authors： Dawe RD,Wright JJ

更新日期：1988-04-01 00:00:00

abstract：:1,2-Diacylglycerol, which has been recognized as one of the intracellular second messengers, was measured quantitatively in the lipid extract from rat hearts using the thin layer chromatography and flame ionization detection (TLC/FID) method. Cholesterol acetate was added to the tissue as an internal standard, and the...

journal_title：Lipids

authors： Okumura K,Hashimoto H,Ito T,Ogawa K,Satake T

更新日期：1988-03-01 00:00:00

abstract：:The subcellular distribution of the enzymes of triacylglycerol biosynthesis has been studied in developing oilseed rape. All in vitro enzymatic activities from oleoyl-CoA to triacylglycerol were sufficient to account for the known rate of oleate deposition in triacylglycerol in vivo. The enzymatic activities from oleo...

journal_title：Lipids

authors： Murphy DJ

更新日期：1988-03-01 00:00:00

abstract：:Analysis of cheek cell lipids has been suggested as a noninvasive method for monitoring the fatty acid composition of diets in humans. In a pilot study conducted to determine the validity of the method, cheek cell samples were collected from subjects consuming a low fat (20% of calories) diet consisting of fatty acids...

journal_title：Lipids

authors： Sampugna J,Light L,Enig MG,Jones DY,Judd JT,Lanza E

更新日期：1988-02-01 00:00:00

abstract：:The production of cardiac arrhythmias and the elevation of lysophosphatidylcholine level in the ischemic myocardium have been well-documented in a number of studies. However, the relationship between the production arrhythmias and the elevation of tissue lysophosphatidylcholine level was not reported. In this study, t...

journal_title：Lipids

authors： Kinnaird AA,Choy PC,Man RY

更新日期：1988-01-01 00:00:00

abstract：:Rat hepatocytes in monolayer culture were utilized to determine if the decrease in glucose-6-phosphate dehydrogenase (G6PD) activity resulting from the ingestion of fat can be mimicked by the addition of fatty acids to a chemically, hormonally defined medium. G6PD activity in cultured hepatocytes was induced several-f...

journal_title：Lipids

authors： Salati LM,Adkins-Finke B,Clarke SD

更新日期：1988-01-01 00:00:00

abstract：:Male and female weanling rats that were born to dams fed a diet low in linolenic acid received diets of 15% lipids by weight containing 45% elaidic acid (as trielaidin) and 8.5% or 0.1% linolenic acid for 10 weeks. Four other groups, in which palmitic or oleic acid replaced elaidic acid in the diet, served as controls...

journal_title：Lipids

authors： Astorg PO,Chevalier J

更新日期：1987-12-01 00:00:00

abstract：:After two months feeding either an (n-3) or an (n-6) fatty acid-rich diet, two groups of trout were switched to the (n-6) or the (n-3) fatty acid-rich diet, respectively. Half of each group was treated with 0.03% 5,8,11,14-eicosatetraynoic acid (ETYA) in the diet. Liver and intestinal brush border membrane lipids were...

journal_title：Lipids

authors： Pelletier X,Leray C

更新日期：1987-12-01 00:00:00

abstract：:SRI 62-834, an analog of the antitumor agent ET-18-OCH3 in which the oxygen atom at carbon atom 2 has been incorporated into a five-membered heterocycle, has been prepared and evaluated as an antitumor agent. The compound exhibited good cytotoxicity in vitro against a variety of tumor cell lines and was as effective a...

journal_title：Lipids

authors： Houlihan WJ,Lee ML,Munder PG,Nemecek GM,Handley DA,Winslow CM,Happy J,Jaeggi C

更新日期：1987-11-01 00:00:00

abstract：:This review covers the work of our laboratory on the antineoplastic activity of some ether lipids and derivatives that are related to platelet-activating factor (PAF). Various 1-O-alkyl lysophospholipid derivatives (ALP) show therapeutic activity in mouse transplant tumor models and in metastatic 3-Lewis lung carcinom...

journal_title：Lipids

authors： Berdel WE

更新日期：1987-11-01 00:00:00

abstract：:The synthesis of thioether phospholipids, which represent a new class of antitumor agents, is reported here. In particular, the route of synthesis of 3-hexadecylmercapto-2-methoxymethylpropyl-2'-trimethylammoni o-ethyl phosphate (BM 41.440, Ilmofosine), one of the most potent cytostatic/cytotoxic derivatives, is descr...

journal_title：Lipids

authors： Bosies E,Herrmann DB,Bicker U,Gall R,Pahlke W

更新日期：1987-11-01 00:00:00

abstract：:1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor, PAF) is known to stimulate platelet aggregation and serotonin release in concentrations ranging from 10(-10)-10(-5) M. Since a variety of synthetic PAF analogues are potent antineoplastic agents in vitro and in vivo, it was the aim of th...

journal_title：Lipids

authors： Söling U,Eibl H,Nagel GA,Unger C

更新日期：1987-11-01 00:00:00

abstract：:The acylation of lysophospholipids by rat liver acyltransferases was studied. A comparison between ester and ether lysophospholipids as substrates revealed large differences in substrate properties. For instance, oleic acid from oleoyl-CoA and arachidonic acid from arachidonoyl-CoA were not incorporated into 1-O-octad...

journal_title：Lipids

authors： Neumüller W,Fleer EA,Unger C,Eibl H

更新日期：1987-11-01 00:00:00